Location: BRB 106
Hours: Mondays and Wednesdays 10am-11am or by appointment
Phone: (406) 243-6013
Dartmouth Medical School Lebanon, NH, 2000-2005 Ph.D. Microbiology and Immunology (Lloyd Kasper, MD)
Immune modulation and the prevention of pathogen-driven TH1 mediated ileitis in mice as a model for the human inflammatory bowel disease, Crohn’s Disease.
Identified and characterized a subset of regulatory CD8+ intraepithelial lymphocytes induce by a novel triterpenoid
Identified mechanisms leading to the activation of TH1 effector cells from the lamina propria of the small intestine
Identified novel plasmacytoid like dendritic cells in the Gut Associated Lymphoid Tissue (GALT) of mice after oral infection with Toxoplasma gondii
University of Montana Missoula, MT (100% self-supported) BS Microbiology, 1997-1999, BA (honors) Psychology, 1993-1997
Lecturer, University of Montana 2010-present: Human Anatomy and Physiology (BIOL312/313), Human Anatomy and Physiology I&II for Health Professions (BIOH365/370), Teaching Anatomy and Physiology/Honors (BIOH 480), Human Anatomy and Physiology Tutor/Honors (BIOB491/BIOH485), Medical Physiology (BIOH460), Independent Research and/or Internships (BIOB 498)
Online Instructor, Major Online Universities beginning in 2009: Anatomy and Physiology (2009), Research Methods in Health Science, Bachelor of Science Capstone (2009), Pathophysiology (2009)
Instructor, Community College of Vermont, fall 2005-Summer 2006: Elements of Microbiology In-class and Online Hybrid (4 semester credit hours)
Laboratory Instructor/Lecture Teaching Assistant, Dartmouth College, 2002: Cell Biology Laboratory (2 sections)
Guest Lecturer, Colby Sawyer College, 2002: Microbiology
Immune Design Corporation, Seattle WA, 2009- June, 2010 Staff Scientist, Project Leader Clinical Immune Monitoring
Design and develop IND-enabling studies for phase I clinical trials for proprietary vaccines at a startup biotech company.
Effectively interface with Clinical Operations, Molecular Biology, Pre-clinical Immunology, Formulations, Regulatory, Quality Control and Quality Assurance Sub teams to adhere to condensed timelines for first in-human vaccines
Perform Assay Transfer and Technology Transfer to Contract Research Organizations
GlaxoSmithKline Biologicals Hamilton, MT, 2007-2009 Post-Doctoral Scientist, Immunology (Melinda Hutton, DVM, Jory R. Baldridge, PhD)
Designed and conducted preclinical immunology studies to evaluate novel vaccine efficacy in mouse models of infectious disease
Characterized cytokine responses from human peripheral blood mononuclear cells (PBMCs), monocyte derived dendritic cells, macrophages and other monocytic cell lines after stimulation with novel TLR agonists.
Determined unique cell signaling pattern in monocytic cells after stimulation with novel compounds using intracellular imaging and Western Blot analysis
Generated stable siRNA knock down monocytic cell lines for use in screening assays
Dartmouth Medical School Lebanon, NH, 2005-2007 Post-Doctoral Associate, Immunotherapy Center at Dartmouth (Randolph Noelle, PhD and Lloyd Kasper, MD)
Evaluated the role of inflammatory T cells (CD4+ TH1 and TH17) cells in patients with Multiple Sclerosis
Performed immunogenicity studies to support a Phase I clinical trial using a humanized monoclonal anti-IL12p40 antibody in subjects with relapsing and remitting Multiple Sclerosis
Performed immunogenicity studies on peripheral blood from MS patients undergoing immunotherapy to determine MOA of approved therapies.
1. Minns, L.A., DuPont, R., Channon J, Noelle RJ, Kasper, LH. In Preparation. Blocking IL-12p40 in Patients with Multiple Sclerosis does not affect peripheral TH1 or TH17 Cells. In preparation.
2. Mielcarz, D.W., Minns, L., Rachinel, N., Menard, L.C., Darche, S., Buzoni-Gatel, D., Kapser, L.H. Polysaccharide A from Bacteroides fragilis modulates pathogen driven ileitis by activating regulatory T cells. In Preparation.
3. Menard, L.C., L.A. Minns, S. Darche, D.W. Mielcarz, D.M. Foureau, D. Roos, F. Dzierszinski, L.H. Kasper, and D. Buzoni-Gatel. 2007. B cells amplify IFN-gamma production by T cells via a TNF-alpha-mediated mechanism. J Immunol 179:4857-4866.
4. Minns, L.A., L.C. Menard, D.M. Foureau, S. Darche, C. Ronet, D.W. Mielcarz, D. Buzoni-Gatel, and L.H. Kasper. 2006. TLR9 is required for the gut-associated lymphoid tissue response following oral infection of Toxoplasma gondii. J Immunol 176:7589-7597.
5. Rachinel, N., D. Buzoni-Gatel, C. Dutta, F.J. Mennechet, S. Luangsay, L.A. Minns, M.E. Grigg, S. Tomavo, J.C. Boothroyd, and L.H. Kasper. 2004. The induction of acute ileitis by a single microbial antigen of Toxoplasma gondii. J Immunol 173:2725-2735.
6. Minns, L.A., D. Buzoni-Gatel, K.H. Ely, N. Rachinel, S. Luangsay, and L.H. Kasper. 2004. A novel triterpenoid induces transforming growth factor beta production by intraepithelial lymphocytes to prevent ileitis. Gastroenterology 127:119-126.
7. Mennechet, F.J., L.H. Kasper, N. Rachinel, L.A. Minns, S. Luangsay, A. Vandewalle, and D. Buzoni-Gatel. 2004. Intestinal intraepithelial lymphocytes prevent pathogen-driven inflammation and regulate the Smad/T-bet pathway of lamina propria CD4+ T cells. Eur J Immunol 34:1059-1067.
8. Kasper, L., N. Courret, S. Darche, S. Luangsay, F. Mennechet, L. Minns, N. Rachinel, C. Ronet, and D. Buzoni-Gatel. 2004. Toxoplasma gondii and mucosal immunity. Int J Parasitol 34:401-409.
9. Luangsay, S., L.H. Kasper, N. Rachinel, L.A. Minns, F.J. Mennechet, A. Vandewalle, and D. Buzoni-Gatel. 2003. CCR5 mediates specific migration of Toxoplasma gondii-primed CD8 lymphocytes to inflammatory intestinal epithelial cells. Gastroenterology 125:491-500.
10. Channon, J.Y., K.A. Miselis, L.A. Minns, C. Dutta, and L.H. Kasper. 2002. Toxoplasma gondii induces granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor secretion by human fibroblasts: implications for neutrophil apoptosis. Infect Immun 70:6048-6057.
Field of Study
Human Anatomy and Physiology, Immunology, Autoimmune Diseases, Vaccine Development
Institut Pasteur Paris, France March 2004-July 2004
Visiting Research Scientist, Department of Parasitology and Immunology (Dominique Buzoni-Gatel, PhD
Characterized the immune response of axenic mice after oral infection with T. gondii
Identified and characterized requirement of Toll Like Receptor in the induction of TH1 mediated inflammation after oral infection with the pathogen, T. gondii
Human Anatomy and Physiology (BIOL312/313), Human Anatomy and Physiology I&II for Health Professions (BIOH365/370), Teaching Anatomy and Physiology/Honors (BIOH 480), Human Anatomy and Physiology Tutor/Honors (BIOB491/BIOH485), Medical Physiology (BIOH460), Independent Research and/or Internships (BIOB 498)